New opportunities for preoperative diagnosis of anaplastic thyroid cancer

S. A. Lukyanov; Лукьянов С. А.; S. V. Sergiyko; Сергийко С. В.; S. E. Titov; Титов С. Е.; Yu. A. Veryaskina; Веряскина Ю. А.; A. M. Mudunov; Мудунов А. М.; V. Z. Dobrokhotova; Доброхотова В. З.; E. S. Kozorezova; Козорезова Е. С.; S. L. Vorobyov; Воробьев С. Л.; A. V. Vazhenin; Важенин А. В.; A. F. Romanchishen; Романчишен А. Ф.; K. V. Vabalaite; Вабалайте К. В.; A. S. Vilkova; Вилкова А. С.; N. I. Timofeeva; Тимофеева Н. И.; T. E. Ilinа; Ильина Т. Е.

doi:10.17650/2222-1468-2021-11-1-34-40

New opportunities for preoperative diagnosis of anaplastic thyroid cancer

Authors: Lukyanov S.A.¹, Sergiyko S.V.¹, Titov S.E.²^,3^,4, Veryaskina Y.A.², Mudunov A.M.⁵^,6, Dobrokhotova V.Z.⁵^,6, Kozorezova E.S.⁷^,8, Vorobyov S.L.⁷, Vazhenin A.V.¹^,9, Romanchishen A.F.¹⁰, Vabalaite K.V.¹⁰, Vilkova A.S.⁵, Timofeeva N.I.¹¹, Ilinа T.E.¹
Affiliations:
1. South Ural State Medical University, the Ministry of Health of Russia
2. Institute of Molecular and Cellular Biology, SB RAS
3. Vector-best, JSC
4. Novosibirsk State University
5. N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
6. I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
7. National Center for Clinical Morphological Diagnostics
8. Institute of Molecular Pathology and Pathomorphology, Federal Research Center for Fundamental and Translational Medicine
9. Chelyabinsk Regional Clinical Center of Oncology and Nuclear Medicine
10. St. Petersburg State Pediatric Medical University, Ministry of Health of Russia
11. Clinic of High Medical Technologies n. a. N.I. Pirogov (Clinic, Hospital), St. Petersburg State University
Issue: Vol 11, No 1 (2021)
Pages: 34-40
Section: DIAGNOSIS AND TREATMENT OF HEAD AND NECK TUMORS
Published: 23.04.2021
URL: https://ogsh.abvpress.ru/jour/article/view/609
DOI: https://doi.org/10.17650/2222-1468-2021-11-1-34-40
ID: 609

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Abstract
About the authors
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Abstract

Background. Anaplastic thyroid cancer (ATC) is one of the most aggressive human tumors. Since median survival of ATC patients is only 4 months, its early diagnosis is very important. Although ATC has specific clinical manifestations, the analysis of expression of different microRNAs can facilitate preoperative diagnostics and help to detect its potential precursors among differentiated cancers and other thyroid malignancies.

The study objective is to identify microRNAs specific for ATC that are different from microRNAs in other thyroid cancers.

Materials and methods. We analyzed the expression of 14 microRNAs in histological specimens of 67 patients with ATC. The control groups included 25 patients with benign nodules, 36 patients with follicular adenomas, 32 patients with follicular cancer, and 152 patients with papillary thyroid cancer. For 7 out of 67 ATC patients, we compared mi-croRNA levels in histological and cytological specimens.

Results. Patients with ATC demonstrated a statistically significant decrease in the expression of miR-145, miR-125b and increase in the expression of miR-155 and miR-21 compared to all control groups. We found two reliable diagnostic markers of ATC: relative miR-21 expression (at a cutoff of 14.9, sensitivity was 0.955 and specificity was 0.837) and the miR-21/miR-145 ratio (at a cutoff of 122, sensitivity was 0.955 and specificity was 0.955). The level of miR-21 expression and miR-21/miR-145 ratio in cytological specimens were accurate in all 7 cases (100 %).

Conclusion. the level of expression of specific microRNAs can be used as a reliable biomarker for ATC. The consistency between the results obtained in cytological and histological specimens enables the use of stained cytological samples for this analysis.

Keywords

anaplastic thyroid cancer, microRNA, diagnostics

About the authors

S. A. Lukyanov

South Ural State Medical University, the Ministry of Health of Russia

Author for correspondence.
Email: 111lll@mail.ru
ORCID iD: 0000-0001-5559-9872

Sergey Anatolyevich Lukyanov

64 Vorovsky St., Chelyabinsk 454048

Russian Federation

S. V. Sergiyko

South Ural State Medical University, the Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0001-6694-9030

64 Vorovsky St., Chelyabinsk 454048

Russian Federation

S. E. Titov

Institute of Molecular and Cellular Biology, SB RAS; Vector-best, JSC; Novosibirsk State University

Email: fake@neicon.ru
ORCID iD: 0000-0001-9401-5737

8/2 Acad. Lavrentiev Ave., Novosibirsk 630090; Bld. 4, 1/1 Arbuzov St., Novosibirsk 630117; 1 Pirogova St., Novosibirsk 630090

Russian Federation

Yu. A. Veryaskina

Institute of Molecular and Cellular Biology, SB RAS

Email: fake@neicon.ru
ORCID iD: 0000-0002-3799-9407

8/2 Acad. Lavrentiev Ave., Novosibirsk 630090

Russian Federation

A. M. Mudunov

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0003-1255-5700

24, Kashirskoe Hwy, Moscow 115522; 3 Tsyurupy St., Moscow 117418

Russian Federation

V. Z. Dobrokhotova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

Email: fake@neicon.ru
ORCID iD: 0000-0001-5889-392X

24, Kashirskoe Hwy, Moscow 115522; 3 Tsyurupy St., Moscow 117418

Russian Federation

E. S. Kozorezova

National Center for Clinical Morphological Diagnostics; Institute of Molecular Pathology and Pathomorphology, Federal Research Center for Fundamental and Translational Medicine

Email: fake@neicon.ru
ORCID iD: 0000-0002-3659-7510

Bld. 2, 8 Oleko Dundicha St., St. Petersburg 192283; 2 Timakova St., Novosibirsk 630060

Russian Federation